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Intra Cytoplasmic Sperm Injection

Intra Cytoplasmic Sperm Injection

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ICSI- Intra Cytoplasmic Sperm Injection

In scientific terms, the natural fertilization process involves the penetration of a single sperm from the ejaculated semen into egg commonly known as oocyte which is released into the fallopian tube during ovulation. It is important that the sperm must penetrate the covering of the egg in order to fuse immediately with the female gamete. However, sometimes either due to biological deficiencies, reduction in the gametes productions, poor gamete quality or due to some fault in sperm and egg interaction, the fertilization process can be prevented and results in infertility.

But thanks to the recent advances in the medical science, we can now overcome these deficiencies by two main approaches in Assisted Reproduction Techniques (ART). These approaches are Conventional In-Vitro Fertilization (IVF) and Intra Cytoplasmic Sperm Injection (ICSI).

Intracytoplasmic Sperm Injection, commonly called as ICSI, is an advanced and most commonly used technique of IVF. In this procedure, your partner’s sperm is injected directly into your egg for fertilization in-vitro. ICSI technique is done to insert a healthy and single sperm directly into an oocyte and thus surmounting the limitations of conventional IVF like poor semen samples, male infertility issues or problems in the interaction between female and male gamete during fertilization.

This procedure is successfully performed with the assistance of an advanced micromanipulation technique, which comprise of a microscope, micro-injectors and micro-pipettes. With the help of these equipment’s, a single healthy sperm is isolated, selected and immobilized. After this, the oocyte is held during microinjection and that selected sperm is inserted and released inside the inner part of the oocyte. After microinjection, each oocyte is continually checked for the signs of successful fertilization.

The ICSI procedure is considered as the revolutionary procedure since it virtually guarantees the successful penetration of sperm into an egg. With the capability to fertilize an egg with just one sperm, the couples facing infertility issue can consider ICSI as one of the promising option to finally become pregnant.


ICSI treatment procedure is a potential option for a couple to conceive especially when they are facing male infertility issue.

Some of these problems are mentioned below:

  • Low Sperm count
  • Poor Sperm Quality
  • Poor Sperm Motility due to which sperm is unable to penetrate and fertilize an egg
  • Any abnormality or blockage in the male’s reproductive tract that affects the normal production or ejaculation of sperms

Other than the male infertility issues, there are several other causes which may lead to infertility in the couples and ICSI can be helpful for them if they have not been successful in conceiving with the IVF in previous cycles.

Enlisted below are few of such conditions :

  • Ovulation disruptions: Disturbance in the ovulation owing to infrequent, irregular or absent menstrual cycle results in lesser number of eggs available for fertilization.
  • Fallopian tube blockage or damage: Blockage or impairment in the fallopian tubes makes it difficult for an egg to get fertilized or for a fertilized embryo to propagate through tube to the uterus.
  • Premature Ovarian failure: It is termed as loss of normal ovarian function before women turns 40 years of age. In such cases, the ovaries fail to produce normal amount of estrogen hormone and to release matured egg regularly.
  • Endometriosis: This occurs with the uterine wall tissues starts growing outside the uterus thereby affecting the normal functioning of ovaries, fallopian tubes and uterus.
  • History of tubal sterilization or removal: Tubal ligation is a type of sterilization procedure wherein the fallopian tubes are either blocked or cut to permanently prevent pregnancy. In such cases, if the female wish to conceive, IVF can be an alternative solution rather than a tubal ligation reversal procedure.
  • Unexplained infertility: In cases where there has been no known cause of infertility in spite of doing all the evaluations, ICSI can be the solution.


Intracytoplasmic sperm injection (ICSI) procedure is an advanced form of IVF which is performed using a specialized technique called as micro-manipulation. This technique uses a special microscope along with other surgical tools like micro-injectors and micro-pipettes to select and handle a single healthy sperm and injecting it directly into an egg.

The steps for ICSI procedure are somewhat similar to the IVF procedure except the phase of fertilization.

The phases for ICSI procedure are described below:

  • Phase 1: Ovarian Stimulation
    Normally, a woman produces one egg during each menstrual cycle. Like IVF, for ICSI as well we need multiple eggs since it increases the chances of developing a viable embryo. In order to achieve that, during the stimulation phase, a woman will be give daily injections of fertility medications for 9 to 11 days. This will boost the egg production and enables the ovaries to produce several eggs in one cycle. During this period, the fertility specialist will perform regular blood investigations and ultrasound so as to monitor the production of eggs and to determine the perfect time to retrieve them.
  • Phase 2: Sperm Extraction
    A sperm sample from your partner can be taken in two ways. Either directly through semen collection or we can extract the sperms surgically from your partner’s testes or epididymis in case of poor sperm count and quality.
  • Phase 3: Egg Retrieval
    Egg retrieval also known as follicular aspirations is small surgical procedure performed under anesthesia. Under the guidance of ultrasound, the surgeon will pass a thing needle through the upper portion of vagina into your ovary and into an egg-containing follicle. This needle will then aspirate the eggs and fluid out of each follicle. The recovery after this procedure is usually rapid and you will be monitored for next 1 hour after the procedure before going home. Some women may experience mild cramping on the day of retrieval which usually subsides by the evening of retrieval.
  • Phase 4: Fertilization of Eggs and Sperm with Micromanipulation
    In this phase, semen sample is taken from the male. The eggs are identified and isolated from the follicular fluid and placed in an incubator. Later, these eggs are fertilized with sperm by Intracytoplasmic Sperm Injection (ICSI) technique. The embryologist picks up a single sperm under microscope and injects it directly into the egg cytoplasm using a small glass needle called as microinjection.
  • Phase 5: Embryo Culture
    This egg is then monitored to document fertilization. Once fertilized, the egg starts dividing and develops into an embryo. The embryologist keeps a close check on embryo to make sure it matures properly. On day two or three after fertilization, the embryos will be evaluated for blastocyst culture. The embryos are then grown for five or six days until they reach the blastocyst stage.
  • Phase 6: Embryo Transfer
    This is a painless procedure in which the embryo is placed into the uterus through a highly specialized catheter under the guidance of ultrasound. No sedation is necessary for this procedure and is usually done 3-5 days after fertilization. After this embryo transfer, progesterone supplementation via injection or vaginal suppository will be given for next 10-12 days and a blood pregnancy test will be performed approximately 2 weeks after the egg retrieval date.
  • Phase 7: Implantation and Pregnancy Test
    A successful pregnancy occurs when the embryo implants itself into uterine wall called as endometrium. This can take 6-10 days after embryo transfer phase. Then the initial pregnancy test is performed to determine if you are pregnant. Once the viability is confirmed, you will be referred the Obstetric and Gynecologist for appropriate prenatal care.

Are you a Candidate?

ICSI is usually indicated in couples presenting alterations in the semen analysis like low concentration and motility of the viable sperms. You can also be considered for ICSI procedure if you are facing ovulatory dysfunction, diminished ovarian reserves, tubal issues, uterine problems, premature ovarian failure, endometriosis and unexplained fertility. ICSI can also be helpful if you have not been successful in conceiving with the conventional IVF in previous cycles.

Expected Results

One cycle of ICSI takes about 4-5 weeks to complete and often boost the success rate of conception for couples facing male infertility issues. The outcome of ICSI is overwhelming since the doctor injects a single sperm directly into an egg which guarantees fertilization of egg and sperm and thereby increases the chances of successful conception.

Recovery Timeline

Recovery timeline for ICSI is similar to IVF treatment. One cycle of ICSI takes about 4-5 weeks to complete. It includes the steps like ovulation induction, egg retrieval, sperm retrieval, fertilization using micro-manipulation and embryo transfer. You would need to stay in India for 4-5 weeks for one cycle, after which the pre-natal care can be continued in your country.

Cost of ICSI Treatment

India is one of the most affordable places for ICSI treatment across the globe. We certainly understand that if you have chosen to travel overseas for your ICSI treatment, then you are indeed looking for cost effectiveness. When you speak about the best, MedicoExperts is the name you can trust on. Modern technology, State of the art hospitals, most coveted gynecologist and fertility specialists, excellent infrastructure at par with international standards, no language barrier, personalized attention, safe surgery and seamless travel are some of our zeniths which magnetizes individuals worldwide to get their Infertility treatment in India done through us.

We are thus committed to offer you the best possible and special negotiated prices for your ICSI treatment without compromising on the quality of medical services and results. Here's a look at the approximate costs of ICSI treatment in India:

SNo Name of Procedure Cost in India (USD)
1 ICSI Evaluation Package 600 - 1000
2 Intracytoplasmic Sperm Injection (ICSI) 3500 - 6000
3 Intracytoplasmic Sperm Injection (ICSI) with Donor Egg 4500 - 7500
4 Intracytoplasmic Sperm Injection (ICSI) with Donor Sperm 4500 - 7500

Why MedicoExperts?

Selecting a medical facility for your fertility treatment can be confusing, stressful and overwhelming. The choice you make will profoundly affect your future and chance for success, and therefore, it is very important that you spend time getting educated before making a choice.

At MedicoExperts, we understand the significance of your health and well-being. Our aim is to provide you the best options and outcomes for your ICSI Treatment in India at our top associate hospitals in India. Our success rates for ICSI treatment put us on the top list in India, giving you the best chance of starting your family. We also understand that while you choose to travel abroad for your ICSI Treatment in India, you would surely look for cost savings and that is why we have special negotiated prices for you and offering you best possible prices for the top quality of facility and services.

We would also provide you end to end assistance for your medical trip to India and shall provide complete solution for your Infertility Treatment- right from assisting you with the visa, hotel arrangements, airport pick up and drop, inland transfers, money exchange or sight-seeing and all other concierge services. We thus assure you hassle-free arrangements for evaluations, surgery, recuperation, stay and travel in India.

For more information and getting a tailor made treatment plan for your ICSI Treatment in India, please mail us your medical history at enquiry@medicoexperts.com.

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